Sentence examples for maximal annealing temperature from inspiring English sources
The cycling conditions applied to the nested PCR were as described above except for the use of a maximal annealing temperature of 69°C and a final touchdown temperature of 59°C.
To overcome bias in methylation analysis (we in fact observed preferential allelic amplification that was annealing temperature dependent) [70], we used various annealing temperatures ranging from 55 to 60°C and employed both Go-taq (Promega) and the Fusion Taq polymerases (Finnzymes), to allow the use of maximal annealing temperature.
Prior to use on the feces DNA all primer pairs were tested on DNA from representative species and PCR conditions optimized with regard to annealing temperature in order to obtain maximal sensitivity.
Using this annealing temperature, band density was found to be maximal at a primer volume of 0.6 μL (10 μmol/L) and a plasmid DNA volume of 1.0 μL.
The Nd3+ PL intensity is then maximal after annealing at 1100 °C which is generally admitted as the optimal annealing temperature for the PL of Si-np. Figure 7 shows the behavior of the PL spectra of the thin films annealed at 1100 °C as a function of the Nd concentration.
The maximal volume of specific surface area at 350°C (Figure 4) indicates that this annealing temperature is more optimal for obtaining powders with minimal particle size and free carbon content.
The annealing temperature was 1,100°C.
The annealing temperature was 750°C.
The temperature represents the nanowire annealing temperature.
Such annealing also crystallizes BN, the size of the crystallites increasing with the annealing temperature.
