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It can provide maximal amplification rate up to 1200 times.
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Under a sigmoidal model, amplification rate is maximal at the onset of thermocycling, but progressively decreases such that each cycle has a unique amplification efficiency, with entry into the plateau phase occurring as amplification efficiency approaches zero.
Central to this is the prediction that the dynamics of PCR amplification can be described by a linear equation, defined here as: (3) E c = Δ E × F c + E max where the slope defines the rate of loss in cycle efficiency (ΔE) and the intercept defines the maximal amplification efficiency (E max ) when F C = 0.
An analytical model for the amplification ratio and the relative amplification rate is established based on the screw theory.
Initially maximal amplification allows cell numbers in PGB, MGB and GRA to grow.
Non-uniform amplification rate results in discarding fragments with insufficient amplification rate (<3).
When phase-amplitude profiles were aligned to maximal amplification, both amplification and suppression were significant, again suggesting a relatively constant anti-phase relationship between amplification and suppression (Fig. 3A).
When the amplification rate is lower, oscillations occur in the same region as without amplification.
Not surprisingly, the food and beverage category proved to generate the highest amplification rates and the highest total media value.
We consider the pathway with the amplification terms at both low and high amplification rates.
Amplification rates and polymorphism are reported in Table 3.
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