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The larger amount of these longer RNA molecules in the brain sample is also reflected in longer maximal amplicon size of 600 nt, compared to 400 nt in kidney (Fig. 4).
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The observed fragmentation also resulted in shorter maximum amplicon size in our RT-PCR system.
Amplicon size was limited to a maximum of 90 bp.
Expected amplicon size was approximately 900 bp.
List of primers used for qRT-PCR analysis and their sequence, and the expected amplicon size.
PCR mixture, primers targeted gene name, cycling parameters amplicon size are shown in Table 1.
LH-PCR was performed on the DNA from the clones, and the amplicon size was recorded as previously described.
Amplicon size was designed to be different for beef, lamb, and pork.
Each qRT-PCR assay was validated by amplicon size and sequence.
The amplicon size for Mtb and Mbo was ∼116 bp and 89 bp respectively [15].
Profile height has also been found to be independent of amplicon size (data not shown).
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