Suggestions(2)
Exact(4)
The enzyme exhibited an optimal activity at pH 5.0 and 40 °C and retained over 20% of maximal activity over the temperature range 0 20 °C.
The enzyme retained more than 50% of its maximal activity over a broad range of pH values (4.5 7.5).
The effect of temperature on the xylan hydrolysis of LrXynA was determined at different temperatures ranging from 30 to 80 °C, at pH 6. LrXynA showed optimal activity at 65 °C, and displayed 50% of its maximal activity over a wide temperature range; from 35 to 70 °C (Fig. 5b).
Norephedrine-based compound 3 and norpseudoephedrine-based 4 had improved maximal activity over 1 and slightly improved potency in the C3H10T1/2 assay, as did the prolinol derivative 5.
Similar(56)
Without any stabilizer, the purified AnSHMT showed significant stability under weakly acidic and alkaline environment (pH 6.5-8.5), retaining over 80% of the maximum activity over a pH range from 7.0 to 8.5 for 24 h at 4°C, and more than 70% of the maximal activity at pH 6.5.
Hence, B. stratosphericus lipase has preferred the high temperature of 50 °C for maximal activity although the lipase was active over a broad range of temperature between 30 and 80 °C.
Similarly, the I249L-SHMT also showed the maximal activity at pH 7.5, and retained over 85% of its maximal activity at pH 8.0.
Incubation at 70 °C and 75 °C for 5 h resulted in over 60%% of maximal activity and 35.5 % of activity was remained after exposure at 85 °C for 2 h, indicating a very high thermostability.
Considering extracellular enzymes of S. aureus usually accumulate over time and maximal activity is detected in overnight cultures (Kiedrowski et al, 2011), this narrow window of Staphopain A activity was unexpected and the reason for the loss of activity at late time points is unknown.
The activity of the recombinant Xyn2 was highest at 60°C and it was active over a broad range of pH (3.0 8.0) with maximal activity at pH 6.0.
Interestingly, it possessed over 50% of its maximal activity at temperature conditions between 0 and 10 °C, suggesting that this enzyme is able to protect crop against PPT injury in cold environment.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com