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Color scale refers to percentage of maximal activity in the image.
The catalyst with 1 wt.% of P and 1 wt.% of B had a maximal activity in desulfurization and denitrogenation reactions.
CoMo/CCA catalyst with 2 wt.% of carbon had maximal activity in HDO of guaiacol and oleic acid thanks to an optimal balance between cobalt content into CoMoS phase particles and their average length.
Therefore, an optimized oxoadenine TLR7/8 agonist that demonstrated maximal activity in the in vitro assays was further assessed in a vaccine study with the CRM197 antigen in a porcine model.
We designed DNAzymes targeting the mRNA of human telomere reverse transcriptase, two of them turned out to have large difference in Mg2+ concentration requirement (1 mM vs. 20 mM) for maximal activity in vitro.
Another interesting biochemical characteristic is the optimal temperature of the β-1,4-endoglucanase Spr Cel8A from S. proteamaculans, with an optimal activity at 40 °C, which maintains 40 90 % of its maximal activity in the temperature range from 15 to 50 °C (Fig. 3c).
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Nikulshin et al. [8] also investigated the effect of Co/Mo ratio and found out that catalysts with Co/ Co + Mo) = 0.35 had maximal activities in 4,6-DMDBT HDS.
The recombinant apple GADs displayed a similar pH profile as shown for GAD activity in the cell-free extracts of apple fruit (Table 1, Figure 3), as well as a similar pH optimum with the majority of plant GADs, and activities with Ca2+/CaM at physiological pH that are as high or higher than their maximal activities in the literature [ 1].
This enzyme already contains approximately 1 equivalent of BH4 per dimer, resulting in half-maximal activity in the absence of added BH4.
As a comparison, emodin displayed half-maximal activity in BG1Luc4E2 cells at 1.3 μM emodin, approximately 5 orders of magnitude weaker in potency than the E2 standard (EC50 18 pM ± 9.4 nM) and was able to induce luciferase to 104±3%3% of maximum E2 activity.
Interestingly, the maximal activity observed in the presence of NR2B sequence was much lower than that in its absence (Fig. 1).
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