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Promoter 2 was induced between 6 and 10 hours of development and reached maximal activity between 14 and 16 hours to decrease thereafter.
Another enzyme (AiiAAI96) retained about 95% of the maximal activity between pH 6.0 and 8.5 at 30°C [ 27].
The pH optimum is 6.5, and the enzyme maintains >50% of maximal activity between pH 3 8.0.
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Differences in maximal activities between the products of the transfected constructs can be attributed to modest differences in the levels of expression of the transfected enzymes.
The MnP peak timing and maximal activity varied between 7 21 days and 100 800 U l-1, respectively.
It exhibits 10 to 50% of the maximal activity at between 30 and 60°C [ 17].
The activity (U l-1) of MnP enzyme produced and the time period it took to reach the maximal activity varied greatly between the selected white-rot fungi.
Dimerization has been used by this flavoenzyme in evolution to achieve maximal activity, a tighter interaction between the protein moiety and the coenzyme, and higher thermal stability.
Interestingly, it possessed over 50% of its maximal activity at temperature conditions between 0 and 10 °C, suggesting that this enzyme is able to protect crop against PPT injury in cold environment.
It also indicates the difference between maximal activity and maximal rest (Relative Amplitude; RA), and provides data on the 10 most active hours (M10) and the 5 least active hours (L5; for detailed information about these measurements, see the following references ).
Hence, B. stratosphericus lipase has preferred the high temperature of 50 °C for maximal activity although the lipase was active over a broad range of temperature between 30 and 80 °C.
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