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The same was observed with AmyB from H. orenii which also required 5%% NaCl for maximal activity and was active at 0 and 25 % NaCl (Tan et al. 2008).
Thus, although disordered or divergent, the carboxy terminus is required for maximal activity and stability.
AgoG2GI requires the divalent cations of Co2+, Mn2+ and Mg2+ for its maximal activity and thermostability.
The Tsc1 and Tsc2 proteins work together as a complex to achieve maximal activity, and recent reports indicate that phosphorylation of either Tsc1 or Tsc2 can lead to regulation of the complex in cell culture [1], [10].
Interestingly, the orthosteric mGlu2/3 receptor agonist, LY379268, displayed an unusually high potency in stimulating GDNF production in the striatum of living mice, with doses as low as 0.25 mg/kg, displaying maximal activity, and doses≥4 mg/kg being inactive.
Heterologously expressed Hyp- O-GALTs required the divalent cation Mn+2 for maximal activity and utilized UDP- galactose solely as the sugar donor (Additional file 1: Figure S3).
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Using the point of maximal activity with and without BSA as 100%, we calculated the first order rate constant by plotting the ln of the appearance of activity against time.
The data were fitted with the Hill function to calculate maximal activity (Vmax) and Km (Origin, version 8.0).
For example, secreted proteins (e.g., phosphatases and proteases) and metabolites (e.g., toxins and antibiotics) are useful only if produced at pHs reasonably near their maximal activity (Peñalva and Arst, 2002, 2004).
Promoter 2 was induced between 6 and 10 hours of development and reached maximal activity between 14 and 16 hours to decrease thereafter.
The purified recombinant enzyme had a pI of 4.5 and exhibited maximal activity at 25°C and pH 7.2.
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