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Only minimal amounts of methyl viologen reduction (less than 1% of the maximal activities) were observed when either the hydrogenase alone or only the three maturases were expressed.
Maximal activities were observed in the range from around 1.5 to 10 mM CaCl2.
Reduced maximal activities were found in variants A27V (38% of wild-type), D241G (48%) and K304E (46%).
Maximal activities were observed with ortho substituents (compounds 2– 12); substituents at the meta and para positions had neutral or negative effects on potency (compounds 13– 26).
It was observed that in the medium with cellulose and elephant grass maximal activities were obtained of 5.84 ± 0.35 IU/mL and 2.45 ± 0.20 IU/mL, respectively, after 96 hours of culture.
Similar(55)
In cases in which saturation was reached at concentrations below 10 nM 17β-estradiol, an average of the maximal activities was utilized to determine percent of maximal activity.
Maps of oscillatory responses in the ±5 Hz frequency band centred upon the 35 Hz wavelet (for evoked GBA) or maximal activity wavelet for each participant (for induced GBA) during the time window of maximal activity were calculated by means of spherical spline interpolations [56].
Around 80% and 400 % of the maximal activity were observed at 90 and 100 °C, respectively (Fig. 3a).
Indane 7 was also prepared, but its potency and maximal activity were poor.
Consequently, the coordinates of these points of maximal activity were used as the centre of the smaller, circular ROIs with a radius of 5 mm.
The plateau phase observed after 24 hours was likely due to a sufficient amount of active Csp-3 to clive the whole substrate, showing that the maximal activity was reached.
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