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Furthermore, the kinetic parameters K m and V max were determined for PepP using three different tripeptide substrates.
The kinetic parameters k M and V max were determined and calculated from the Nonlinear least squares regression applied to the Michaelis and Menten (http://statpages.org/nonlin.html).html
To confirm the type of inhibition, kinetic constants (K m and V max) were determined using different inhibitor concentrations (10, 15 and 20 mM) of xylose at varying pNPX concentrations (0.23 5.52 mM) under standard assay conditions.
The K m and V max were determined at 85°C by recording the effect of various concentrations (0.05%, 0.1%, 0.15%, 0.2%, 2.5%, 3%) of beechwood xylan.
Taking experimental errors into account, similar values for A max were determined as in adsorption isotherm experiments and, thus, complete saturation of α-cellulose was reached in kinetic studies.
The K m and V max were determined at 28°C by recording the effect of various concentrations (10 to 40 mmol/L) of ABTS and DMP separately [ 42].
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An upper bound λ max is determined as follows.
Values of Langmuir isotherm constants K and W max are determined from the plot.
Please note that in Algorithm 1, running L times of problem (12) is activated after τ min and τ max are determined.
The dominant parameters' boundaries θ min and θ max are determined by their empirical values, so as to guarantee the reasonability of the results.
The maximum power density (the peak in a power curve, P max) was determined based on the projected area of anode (mW m−2).
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