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The samples were cooled on ice for 5 minutes and spun at max speed in a microcentrifuge for 5 min. The aqueous layer was transferred to a fresh tube, an equivalent volume of phenol chloroform:isoamyl alcohol (25∶24∶1) was added, the mixture vortexed for 4 minutes, and then spun at max speed in a microcentrifuge for 5 minutes.
The precipitate was harvested at max speed in a microfuge for 30 minutes at 4°C, and the pellet washed with 1 mL of 70% ethanol, then dried, and resuspended in water.
The samples were heated at 65°C for 10 minutes, vortexing every minute, then cooled on ice for 5 minutes and spun at max speed in a microfuge for 5 minutes.
Heat-treated samples were spun at max speed in a table-top centrifuge.
Samples were spun down 5 min at max speed in a microcentrifuge, and the absorbance at 420 nm was then measured.
Reactions were incubated at various temperatures for 1 hr followed by a spin at max speed in a tabletop centrifuge for 10 min immediately prior to data collection to remove any trace aggregation.
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A group of planarians was also cultured in the previous conditions (max speed 60°/s), in order to reproduce the lethal effects.
It offers standard-def video and max speed of 3Mbps in addition to unlimited talk, text and data (also with slowed speeds after 22 GB of data).
Pellets were resuspended in Trizol, transferred to a tube containing Lysing Matrix B (QBiogene, Inc ., and vigorously shaken at max speed for 30 sec in a FastPrep 120 homogenizer (Qbiogene) three times, with cooling on ice between steps.
Max speed is 80 mph and max range is 80 miles.
Right before the RNA isolation, we spun down antennae preserved in Trizol at the max speed (13,000 rpm) for 3 min at 4°C and transferred each sample into one MagNA Lyser Green Beads tube (Roche).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com