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However the cauline stems of these two max mutants were not significantly stunted as in sk156.
max mutants were kindly provided by Dr. M-S Peng (University of Guelph) and transgenic Arabidopsis lines over-expressing miR156b under the control of the CaMV35S promoter were provided by Dr. R. S. Poethig (University of Pennsylvania).
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MAX2, proposed to act in strigolactone perception, participates in an SCF complex that locally suppresses shoot branching and accordingly, the shoot branching phenotype of max mutants is caused by increased auxin transport capacity in the main stem [ 29, 38, 39].
These findings are broadly consistent with results of previous translation assays of poliovirus mutants differing in CP choice in which relatively small differences in translation of PV-Min, WT and PV-Max mutants were clearly incompatible with the marked differences in their replicative ability (Coleman et al., 2008).
At-max mutants are bushier than wild-type plants, due to outgrowth of axillary meristems that are usually suppressed.
Since the axillary buds of max4 mutants are resistant to the inhibitory effects of apically supplied auxin, auxin and the MAX-dependent hormone must interact to inhibit branching.
By contrast, at 32 °C, all values in the tcd10 mutants (0.559 ± 0.012 for Fv/Fm and 157.200 ± 1.131 for rETR max, respectively) were nearly similar to WT levels (0.642 ± 0.024 for Fv/Fm and 143.800 ± 8.707 for rETR max, respectively), showing that both PSII activity and photosynthetic electron transport in the tcd10 mutants were nearly recovered to WT levels at 32 °C.
Other mutants were generated similarly.
The New Mutants are the outcasts.
Fortunately, these mutants are rare.
A link between altered carotenoid profiles and branching was previously observed in max mutants [ 17, 19] and in an Arabidopsis histone methyltransferase (SDG8 -defective mutant [ 36].
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