Thus, maize leaf data with different developmental stages are generated from mRNA isolated from four developmental zones: basal zone, transitional zone, maturing zone, and mature zone.
The second macroscopic zone (proximal zone) is composed of state B (presence of a blocked primordium or of an emerged lateral root) and state C (root segment between two lateral organs) and corresponds biologically to the mature zone of the root.
(3) Expression of CDKs Genes in Mature Zone of Normal and Endoreduplicated Roots.
Comparative analysis of CDKs gene expression level in apical, elongation, and mature zone of normal, endoreduplicated, and depolyploidized roots was generated using automated ABI 7500 real-time PCR.
Further, CDKs (CDKA 1, CDKA 2, CDKB2 1, CDKB2 2, CDKD1 1, and CDKD1 3) gene expression analysis was done from the three different zones (apical zone, elongation zone, and mature zone) of normal and endoreduplicated Allium cepa root using RT-PCR.
A couple of O2 needle microsensors (OX50, Unisense, DK) have been horizontally inserted at two different levels for each vial, corresponding to the root apex and to the mature zone of the seedling root.
Normal and endoreduplicated roots of Allium cepa were dissected into three zones: (1) apical zone, (2) elongation zone, and (3) mature zone.
Arabidopsis PLL4 (a member of PP2C class) was reported to play a part in the regulation of leaf development, while its homolog in maize (ZmPP24) showed higher expression in the maturing and mature zones as well.
After 12 h (tomato) or 4 h (Arabidopsis) incubation, GFPE. coli and GFPyeast were detected in root hairs and the rhizodermis and cortex of mature zones of the roots (GFPE. coli, Figure 1A D; yeast, Figure 1E F) by confocal laser scanning microscopy (CLSM).
The data for the 10 genes with detectable transcript in maize B73 stem tissues including internode meristematic tissue, rind, vascular bundles, elongating, transition and mature zones of the internode and nodal plate have been extracted.
