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They showed that the donor cells were located within the wound site and ACL exhibited almost normal histology, with more mature spindle cells with higher levels of transforming growth factor (TGF-β) in the BMC group.
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Because the klp10A RNAi knockdown spindles do not shorten as in wild type, klp10A may have a role in shortening and reorganization of microtubules to form the mature MI spindle.
Regardless of origin, mature metaphase spindles are identical and indistinguishable from the typical anastral spindle of higher plants with broad polar regions consisting of numerous subsets of converging microtubules.
We next assessed whether Bace1 activity is needed to sustain mature muscle spindles.
Despite their diverse origin, the mature metaphase spindles are remarkably alike in all three taxa (Fig. 4).
Upon formation of a mature metaphase mitotic spindle the APC/C ubiquitinates securin.
Although Ago2-deficient oocytes can develop to morphologically normal mature oocytes, the spindle and the organization of the chromosomes are abnormal.
The longer stage 14 spindles are presumably not seen in oocytes produced by klp10A P mut/+ heterozygous females because they express enough KLP10A from their wild-type allele to remodel the longer spindles observed in late stage 13 into normal-appearing mature stage 14 spindles.
Interestingly, the morphology of tumors derived from shMET RH30 cells also resembled the morphology of the more mature and differentiated, spindle-shaped SMS-CTR cells.
It is possible that most of the increase in PKC activity that has been reported at fertilization is due to non-conventional and novel isoforms such as PKCζ and PKCδ, which are localized to the meiotic spindle in mature mouse eggs where they may play a permissive role in meiotic resumption (Tatone et al., 2003; Viveiros et al., 2003).
We therefore examined Meiosis I spindles in mature WT and Dgp71WD /Df(2L)ED119 oocytes stained to reveal the distribution of D-TACC — a marker for the acentrosomal poles of the meiosis I spindle (Cullen and Ohkura, 2001) — α-tubulin and DNA.
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