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Similar modification at their 5'-end were detected in A. albopictus miR-252 (35% of mature reads) [ 31].
Resulting in a final 388 precursor sequences, of which 232 where found to have both star and mature reads.
For miR-252 identified in this study, we found 99% of mature reads with a template-directed cytosine addition at the 5'-end.
The final output from miRDeep analysis was a list of putative miRNA precursors with their corresponding 5p and 3p mature reads that was assigned a miRDeep score (score estimated as described in [ 16]).
Interestingly, the largest number of reads that perfectly matched the mature reads from the clustered ssa-miR genes 212 and 132 was observed in brain tissue (sample 10, Table 1).
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With a female protagonist (tick!), clever use of writing style (tick tick!) and some heart-pounding scenes (tick tick tick!), this is a fantastic, mature read to follow The Hunger Games.
Another option is to buy back shares, a proven way to deploy cash, though doing so sends a signal that they are a mature (read: not growth) company.
Particularly when it comes to (gasp) more "mature" (read: sexy, titillating, very slightly dirty) content.
These miRNAs had relatively high mature read counts (Additional file 11: S4).
Of these, pal-can-134 had mature/star read counts of 31/1; however only a single mature read was detected for pal-can-411.
Wheeler and colleagues [ 21] documented evolutionary stable shifts to the determination of position 1 of the mature sequence that can be displaced towards either the 5' or 3' end, a phenomenon called seed shifting, as well as the ability to post-transcriptionally edit the 5' end of the mature read, changing the identity of the seed sequence and possibly the repertoire of downstream targets.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com