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In sugarcane, the accumulation of sucrose in the maturing and mature internodes of the stem contribute greatly to sink strength [27].
In our study, LEA and dehydrin were elevated in the mature internodes of Q117 when compared to the other tissues.
Mature internodes of all three genotypes showed decreased expression of cell wall metabolism-associated genes and increased expression of genes related to sucrose metabolism.
Of the 51 genes tested, 17 genes showed higher expression in the most mature internodes of the culm than in the leaf.
Transcript expression levels were standardized to transcripts of GAPDH as a reference gene because this gene is known to be expressed at a relatively constant level in leaves, and immature and mature internodes of cultivar Q117 [ 16].
A DEHYDRIN TYPE 1 (SCQGLregulatedg) regulated by the DREB signaling cascade [ 64] was dramatically repressed in mature internodes of high and low Brix plants and induced after 72 h and 120 h drought treatment (Table 1).
Similar(51)
The fact that the fibre bundles are in the process of formation very close to the SAM, led many who sampled more mature internodes to think that they are formed by the primary meristem as bundles and to overlook the process of their formation by the gradual association of fibres to bundles via intrusive growth.
Using a sub-set of eight genes, expression was examined in mature internode tissues of sugarcane cultivars as well as ancestral and more widely related species with a range of sucrose contents.
Sucrose content in the mature internodes reach around 20% of the culms dry weight.
This suggests that the increased level of expression of TF1 reached in mature internodes may either be required for increased regulation of sucrose accumulation or be a response to it.
Sucrose content in the mature internodes can reach around 20% of the culms dry weight while lower sucrose levels are observed in younger internodes where glucose and fructose are predominant.
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