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As a whole, four cycles of selection on SM2-OVA, as well as one round of affinity maturation were performed.
Confocal analyses of phagosome acidification and maturation were performed.
The stages of sexual maturation were performed with a questionnaire and physical exam done by an experienced physician as follow: premenarcheal with no secondary sexual development (stage 1).
Egg chamber staging and analysis of oocyte meiotic maturation were performed as described (Von Stetina et al. 2008), with some modifications.
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This can be explained by the fact that AFM imaging in air was performed with mature lithostathine whereas this maturation was performed in the buffer bathing the tip with HS-AFM.
One cycle of affinity maturation was performed.
The evaluation of mammary gland maturation was performed on five of each PyMT negative Mena WT, Het, and Null mice at 6 and 10 weeks of age.
DC were pretreated with LF (5-10 μg/ml) beginning at day 4. Activation of DC maturation was performed by addition of TNFα/LPS for 24 hours.
Pubertal maturation was performed by self-assessmen, using specific figures for breast stage (B1 5) for girls and male genitalia (G1 5) for boys according to the criteria of Marshall and Tanner [ 15, 16].
In vitro maturation was performed with different concentrations of 1% high molecular weight HA (Croma Pharma GmbH, Austria), with 75 μL/2 mL medium (a final concentration of HA 0.035%) and 150 μL/2 mL medium (a final concentration of 0.07%).
Monocyte maturation was performed in RPMI 1640 containing 2% or 10% FCS (as indicated in the figure legends) and 20 ng·monocyteocolony-stimulatingating factor (MCSF) for 24 and 72 h followed by 18 h in serum-free RPMI 1640 to collect cell extracts and conditioned medium.
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