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The experiments were based on freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined.
The effects of Hdac3 depletion on skeletal maturation were determined by crossing Hdac3fl/− mice [11], [31] with mice expressing Cre recombinase from the osterix (Osx) promoter [30].
Tissue-specific changes in endogenous ABA content during fruit maturation were determined.
IBMP concentrations of berries during maturation were determined according to the method of Koch [ 92].
The expression levels of the PDI family proteins, calreticulin, and Ero1 in the caryopses during maturation were determined by western blot analyses.
The effects of anthroposophical medications on DC maturation were determined by measuring surface receptor expression (anti-human CD14, CD83 and CD86 mABs; all from ebioscience, Frankfurt, Germany) using live cell gating in flow cytometric analysis.
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Nuclear maturation was characterized by the presence of a first polar body (metaphase II) at 24 and 48 h after IVM, and cytoplasmic maturation was determined by extent of early embryo development following chemical activation.
Briefly, DC maturation was determined by using fluorochrome-conjugated antibodies that recognize following surface molecules: CD11c, MHC Class II, CD86, and CD40 (mouse, eBioscience) and CCR7, CD80, CD83, CD86, and CD40 (human, eBioscience).
Biofilm maturation was determined and monitored using HPC bacteria enumeration.
Pubertal maturation was determined through standardized methods based on Tanner staging (Biro et al. 2010).
DCs were infected as described above, and phenotypic maturation was determined 24 h after incubation with pneumococci.
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aging were determined
curing were determined
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maturation were done
maturation were detected
maturation were reported
maturation were revealed
maturation were investigated
maturation were generated
maturation were performed
maturation were regulated
maturation were analyzed
maturation were identified
maturation were showed
maturation were documented
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