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Cell distribution, proliferation, organization, and maturation were assessed on each scaffold type in vitro, and engraftment and healing of full thickness wounds in athymic mice were tested.
For this, expression of surface receptors and intracellular proteins involved in phagocytosis and phagosome maturation were assessed in WT, IFNβ−/− or IFNAR1−/− primary microglia as well as the microglia derived, immortalized cell line BV2.
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Cardiomyocyte maturation was assessed through the fetal gene program where expression for atrial natriuretic peptide decreased and sarco endo)plasmic reticulum Ca2+ increased with culture time for the protein-modified surfaces, indicating a trend towards maturation, while the α/β-myosin heavy-chain ratio remained near fetal expression levels for all surfaces.
DC maturation was assessed by changes in an extensive membrane phenotype.
Follicular maturation was assessed by serial (every 2 3 days) transvaginal ultrasound scan and serum estradiol measurements [27].
Sexual maturation was assessed in terms of Tanner stage.
Half were cultured for 24 hours and their maturation was assessed.
Scar maturation was assessed by examining collagen fiber thickness and alignment.
DC maturation was assessed by flow cytometry and cytokine production measured by ELISA.
Follicular maturation was assessed with respect to T/B-cell segregation, CD45RO and CD45RA, CD21+ FDCs, GC and MECA79+ HEVs.
Sexual maturation was assessed by Tanner stage [ 38] and onset of menses was ascertained annually during the DISC trial.
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