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After staining, 20,000 events were acquired on a Becton Dickinson (Mountain View, CA) FACSCalibur® and the expressions of the markers of maturation were analyzed on CD11c-gated cells using CellQuest software.
Effects of these mutants on complex maturation were analyzed by Western blot of cell lysates and densitometry.
To understand accumulation of oil and lignan, and determine time of sampling developing seeds for EST sequencing, two sesame (S. indicum) cultivars Zhongzhi 14 with high oil content and its contrast Miaoqian with low oil content and early maturation were analyzed for accumulation of oil, fatty acids and lignans.
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A minimum of 100 cells at a given stage of maturation was analyzed for HuR and MVH staining.
* percentage of the total CD19+ cells, ** immature cells, ***intermediary and ****mature B-cells B-cell maturation was analyzed in two four-color combinations (Table 6).
Maturation was analyzed in groups of oocytes that had been injected 72 h earlier with as-STIM1 or as-STIM2, and they were compared with uninjected oocytes or those injected with H2O.
Oocyte maturation and embryo development were analyzed by one-way ANOVA, and Tukey's HSD was used as post hoc tests.
A wholly different scenario was evidenced when CD8+ T cell maturation patterns were analyzed in ASCT recipients and in HIV-infected subjects (Fig. 2).
To address DC maturation, the cells were analyzed for CD83, CD86 and MHC class II expression.
Maternal and cord lymphocyte subsets as well as B-cell maturation in cord blood were analyzed by flow cytometry.
Phenotype and maturation state of DCs were analyzed by staining with the following antibodies against PD-L1 (clone MIH1), PD-L2 (clone MIH18, both from Becton Dickinson, Franklin Lakes, NJ, USA) and isotype control IgG1 (Dako).
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