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For quantification of Lat B effect, the length of the embryo at the end of maturation was used as a simple substitutional criterion of embryo maturity.
The MT103 strain, which evades the phagolysosome maturation, was used as negative control and heat-inactivated MT103, which follows the classical phagocytosis route, was used as positive control.
This experimental system, in which expansion is uncoupled from maturation, was used to examine the mechanisms controlling the proliferative responses of proerythroblasts to Epo or SCF.
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Oocytes that were treated with UV light for 30 mins immediately after maturation were used as a positive control.
Recently proposed local cut-off points of homeostasis model assessment index (HOMA), according to sex and pubertal maturation, were used to classify IR [ 23].
During sampling periods two and three, a number of males had matured; therefore, maturation status was used as an additional fixed effect for those sampling periods.
HPRT, which was not regulated during the maturation process, was used as the reference gene.
For each sex, the age distribution (in decimals) of each sexual maturation stage was used to define tertiles of age.
A GFP (green fluorescent protein) mutant with fast maturation time was used as the output reporter to indicate the levels of these chemicals.
For construction of CP1 mutated library, the gene of the CP1 scFv clone, selected from the first maturation library, was used as template for PCR amplification.
A sigmoid maturation model was used to describe changes in M (CLPO + CLPM), M1 formation (CLPM) and M1 elimination (CLMO) clearance.
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