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After that, nuclear maturation was evaluated using orcein staining.
The SNs were characterized by immunocytochemistry and their functional maturation was evaluated by electrophysiology.
MoDC phenotypic maturation was evaluated by extracellular staining with anti-CD83-FITC and anti-CD86-APC (BD Biosciences).
Pubertal maturation was evaluated via self-report and classified according to Tanner [ 43, 44].
The yield of embryos after four weeks of maturation was evaluated using 10 clusters of embryogenic culture in each variant.
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Body weight and lung maturation were evaluated at 125 days.
Osteoblast differentiation and maturation were evaluated using alkaline phosphatase specific activity as an early marker and osteocalcin secretion as a later marker [ 26].
Dendritic spine density and maturation were evaluated by immunocytochemistry via the presence of drebrin (an actin-binding protein) with GluN1 (NMDA receptor subunit) and GluA2 (AMPA receptor subunit) clusters.
Processed data from all 8 mRNA expression profiling experiments are provided in Additional file 2. Differences in the expression levels of each mRNA and miRNA between secretory- and maturation-stage tooth development were evaluated using two-tail Student t test, and the type I error was controlled using the Benjamini Hochberg (B-H) method.
The expression of a panel of maturation molecules was evaluated at several time points after infection.
Previous studies reported a higher impairment of migration not only in MVA-infected MDDC but also in bystander MDDC, maybe due to the lower times of maturation period (migration was evaluated 24 h post-infection) [43], thus probably not giving enough time to MDDC to fully maturate and to undergo upregulation of CCR7.
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processing was evaluated
development was evaluated
age was evaluated
aging was evaluated
maturation was promoted
maturation was indicated
maturation was blocked
maturation was elucidated
maturation was revealed
maturation was tested
maturation was observed
maturation was defined
maturation was used
maturation was stimulated
maturation was triggered
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