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Briefly, DC maturation was determined by using fluorochrome-conjugated antibodies that recognize following surface molecules: CD11c, MHC Class II, CD86, and CD40 (mouse, eBioscience) and CCR7, CD80, CD83, CD86, and CD40 (human, eBioscience).
Nuclear maturation was characterized by the presence of a first polar body (metaphase II) at 24 and 48 h after IVM, and cytoplasmic maturation was determined by extent of early embryo development following chemical activation.
Biofilm maturation was determined and monitored using HPC bacteria enumeration.
Pubertal maturation was determined through standardized methods based on Tanner staging (Biro et al. 2010).
DCs were infected as described above, and phenotypic maturation was determined 24 h after incubation with pneumococci.
20 Biofilm maturation was determined and monitored using heterotrophic plate count (HPC) as outlined in APHA standard method 9215B.
Similar(49)
The experiments were based on freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined.
The effects of Hdac3 depletion on skeletal maturation were determined by crossing Hdac3fl/− mice [11], [31] with mice expressing Cre recombinase from the osterix (Osx) promoter [30].
Tissue-specific changes in endogenous ABA content during fruit maturation were determined.
IBMP concentrations of berries during maturation were determined according to the method of Koch [ 92].
The expression levels of the PDI family proteins, calreticulin, and Ero1 in the caryopses during maturation were determined by western blot analyses.
More suggestions(15)
maturation was established
maturation was demonstrated
ripening was determined
aging was determined
maturation was promoted
maturation was elucidated
maturation was indicated
maturation was blocked
maturation was evaluated
maturation was observed
maturation was stimulated
maturation was triggered
maturation was preserved
maturation were determined
maturation was induced
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