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Hence, its level of expression can also be used as an additional maturation marker for LPS induced chicken dendritic cell maturation along with CD83 and CD40.
CD161 is a marker commonly found on NK cells, and a maturation marker for NKT cells [31].
Berzins et al. describe that NKT cells from thymus were CD161-, in contrast with adult peripheral blood, suggesting that CD161 expression is also a maturation marker for NKT cells in humans[34].
It has been reported that Lymphocyte antigen 6 complex, locus I (LY6I), a maturation marker for T and B lymphocytes [34] is induced during murine tuberculosis [17], and we show that transcripts encoding LY6I is induced during the early immune response on D15, with progressively more induction of D21 (Table 5).
Most transfected DCs expressed high levels of CD83 (a maturation marker for DCs), HLA-DR, CD80 and CD86.
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In this regard, CD45 is a late maturation marker of HSCs whereas CD41 is an early maturation marker of HSCs.
The observed decline of pGE was independent of the maturation marker used for isolating the subsets, i.e. CD80 or MHCII.
miR-124, miR-129, miR-202, miR-203, miR-302b and miR-467a were stably expressed at two- to tenfold higher level in the mTEChigh subset independent of the maturation marker used for sorting the cells (Fig. 1C).
Maturation markers of MDDC were examined by flow cytometry.
Most importantly, the expressions of the genes encoding for the early maturation marker TRAP (Acp5), the most plausible PLCγ2 effector NFATc1 (Nfatc1) [ 11, 31] and of DC-STAMP (Tm7sf4), a critical player of the preosteoclast fusion machinery [ 37, 38], were all upregulated normally in PLCγ2−/− cultures (Fig. 4b).
In addition, we observed cells positive for CD83, a DC maturation marker, more frequently in the peritumoral lung tissue as compared to the NSCLC sections.
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