GPAT maturation was confirmed to be impaired at 4 weeks in Alb-Cre-Fxn L3/L− mice by western blot (Fig. 3E).
Maturation was confirmed by assessing increases in the immunofluorescence of CD80, CD83, CD86 and human leukocyte antigen-DR (HLA-DR), as well as variations in CD1a.
This observation is confirmed by a significant correlation between actinobacteria and maturation phase according to PCA (Fig. 4a, b).
Consistent levels of maturity and responsiveness to maturation signals were confirmed for each DC population by flow cytometric staining for CD11c, CD86 and MHC class II with or without prior LPS treatment.
MDDC (7 day old) were stimulated with LPS (Sigma-Aldrich) 1 µg/ml, and maturation of MDDC was confirmed by phenotypic and functional analysis (Fig. S1A, B).
Efficient maturation of HA was confirmed by the progressive attainment of EndoH-resistant oligosaccharides that occurs when native HA traverses the Golgi compartment (Fig. 2C, lanes 1 6).
Seven differentially expressed miRNAs (miR-21, miR-31, miR-488, miR-153, miR-135b, miR-135a and miR298) in secretory- and/or maturation-stage enamel organs were confirmed by in situ hybridization.
Maturation of the Env-specific antibody response was confirmed by a change in avidity and conformational dependence.
The diagnosis of VA was confirmed by a vaginal maturation index (VMI) containing ≤5% superficial cells and vaginal pH > 5.0.
The maturation of both cell types within the hydrogels was confirmed by cardiac-specific biomolecular markers, including alpha-sarcomeric actin, actinin, and connexin-43.
