The highest meiotic maturation (70.2%) was observed in oocytes cultured in the control medium, and the lowest maturation was in medium supplemented with 0.07% HA (63.1%) (Table 1).
Pooled oocytes were matured in maturation medium for 24 h in 200 µl within a Nunc 4-well dish (Nunclon, Roskilde, Denmark), then inseminated in BIVF medium (200 µl, 2 × 10 / ml final sperm concentration).
As a control, we used COCs matured only in maturation medium and cocultured with granulosa cells [ 18].
In Experiment 4, BSA was used in lieu of serum in the maturation medium in a 12 + 12 h maturation system.
To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium.
The present experiments compared the maturational and developmental competence of immature oocytes derived from stimulated cycles, following culture in a newly designed in-vitro maturation medium (IVM-medium) or in standard tissue culture medium (TCM-199; control).
Soybean somatic embryos of each individual line that were grown for 3 weeks in maturation medium were homogenized in 1 mL Trizol reagent, transferred into microcentifuge tubes, and incubated for 5 minutes at room temperature.
Oocytes with even, dark cytoplasm and more than two compact layers of cumulus cells were selected, washed twice in in vitro maturation medium (IVM) and placed in groups of 40 60 in a final volume of 500 μl of IVM.
Considerable estradiol accumulation was observed in the control maturation medium for in vitro maturation of bovine COCs during the 24 h of maturation (P<0.05).
Slight estradiol accumulation was observed in maturation medium containing progesterone at concentrations of 2.5, 5.0 and 10.0 μg/ml, but these increases were less (P<0.05) than those observed in the control medium.
