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Binary masks of skull, gray matter, and white matter were also generated after boundaries of scalp, skull, gray, and white matters were extracted from MR images, as shown in Figure 4b.
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These 8-mm-diameter spheres were shifted within individuals to ensure that only data from gray matter were extracted.
Masks of whole brain grey and white matter were extracted and the Desikan-Killiany Atlas in Freesurfer was used to obtain 34 grey matter volumes of interest per hemisphere in the native coordinate system of each subject (Dale et al., 1999; Fischl et al., 1999).
In brief, the freeze-dried <63-μm fraction of the suspended matter was extracted with toluene using Soxhlet extraction.
For each solvent, extraction was conducted until no more coloured matter was extracted.
Most matter was extracted with the polar solvents ethanol and water, while little was extracted with the non-polar solvents DCM and ethyl acetate.
The water-soluble soil organic matter was extracted from each sample and fluorescence and UV vis spectroscopy was used to analyze its chemical character.
Adsorption is the phenomenon in which matter is extracted from one phase and concentrated at the surface of a second phase.
Water-soluble organic matter was extracted by shaking pre-incubated soil (adjusted to 50% water holding capacity and incubated for 24 h) at a soil/water ratio of 1 2 on an end-to-end shaker at 20°C for 1 h.
Hot water-soluble organic matter was extracted from these soils by adding water to the wet soil remaining in each tube to return the water volume to 150 ml, then by placing the tubes in a water bath at 80 °C for 16 h.
Organic matter was extracted from filters by ultrasonification with toluene and analyzed using gas chromatography/mass spectrometry.
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