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It is unclear at this moment whether the expression of the remaining Beclin 1 allele is activated in our MCF7 cells during passages or it is simply a matter of assay sensitivity.
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The sensitivity of animal species (mouse versus rat), strain (inbred versus outbred) has been a matter of concern when selecting assay type for testing of estrogenicity of chemicals.
It is matter-of-fact.
It was matter-of-fact.
Woods explains matter-of-factly.
The precision of subtyping assays is a matter of importance and debate for epidemiologic and, recently, forensic investigations.
As such, SARMs have been a matter of preventive doping research, and first assays allowing for the analysis of arylpropionamide-derived SARMs (such as Andarine and Ostarine) were published in 2006.
Thus no matter which assay was used, the compounds exhibited the same activity trends and SAR (Figure2), validating the reliability of both techniques.
As a matter of fact, the specificity of the available assays can vary widely [ 42].
The optimal way to measure antigen-specific T cells remains a matter of debate, especially since recent observations suggest that different assays might measure different T-cell responses.
Nevertheless, choosing the right assay to monitor and asses autophagy has been a matter of debate [ 30, 48, 49].
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