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To improve the separation of organelles, we used a 10 000×g supernatant instead of the 100 000×g pellet as starting material and we noted that iodixanol was superior to sucrose in this matter (data not shown).
There was almost no immunoreactivity to enterokinase in the deep white matter (data not shown).
The residents have low socioeconomic status, living together with domestic animals and accumulated organic matter (data not shown).
CTGF mRNA expression was scanty and observed only in a few normal cells in the deep layers and in the white matter (data not shown).
In normal cerebrum, there was minimal FABP7 reactivity in the white matter (data not shown), and only scattered, predominantly nuclear FABP7 immunoreactivity in the cortex (Fig. 1A).
The raw material before pre-treatment had a xylose content of 24.9 ± 0.4% and a glucose content of 36.1 ± 0.1% dry matter (data not shown).
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We observed fine synaptic PrPd immunoreactivity with focal patchy or plaque-like appearance in gray matter structures, but we also noted ependymal, subependymal, perivascular, and white matter PrPd deposits (data not shown).
At this early time-point of recovery, we did not observe any change in staining for the oligodendrocyte marker Rip within the subcortical white matter or SVZ (Data not shown [26]).
Marked differences were not observed in HK2 and PK immunoreactivity between control white matter and NAWM (data not shown).
Inflammatory cells were found in PML controls in grey and nondemyelinating white matter as well (data not shown).
No marked differences were observed in PDH, αKGDH and MDH immunoreactivity between control white matter and NAWM (data not shown).
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CEO of Professional Science Editing for Scientists @ prosciediting.com