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The binding was also quantified using a reference tissue model with white matter as reference.
The SRTM and reference Logan graphical analysis with cerebellum grey matter as reference region were applied to the regional TACs to estimate the BPND, used to quantify the amount of tracer binding to the target proteins.
These results indicated that reference tissue models using the white matter as reference tissue can be useful for quantification of AMPA receptors; however, this methodology will need to be validated using PET ligands for AMPA receptors with higher amounts of specific binding than [11C]HMS011.
In addition, most clinical studies published to date have used simplified analysis approaches with cerebellum grey matter as reference region.
For subjects with these diseases, the use of white matter as reference region would require further validation and careful investigation.
First, BPND was derived for ROI data using the simplified reference tissue method (SRTM) [ 25] with cerebellar grey matter as reference tissue.
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Form may matter as much as quantity.
Reforms matter as much as deficit targets.
The performance in classifying subjects by discriminant analysis using the subcortical white matter as the reference region was similar to that using the cerebellum as the reference region.
The rank order of DVR values derived using the white matter as the reference region agrees well with that using the cerebellum as the reference region and is consistent with the typical rank order of amyloid deposits seen at autopsy.
This assumption was checked using the same model evaluation strategies adopted by Wong et al. [ 6], and it was found that SRTM outperformed the full reference tissue model in 85% of model fits using the white matter as the reference region (data not shown).
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