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In addition, both cells and matrix were stained throughout the fibrotic regions of the synovium.
Briefly, the ethanol-fixed cells and matrix were stained for 1 h with 40 mM Alizarin red-S (pH 4.2) and extensively rinsed with water.
Highly sulphated mucins, characteristic for cartilage-like matrix, were stained with alcian blue 8GX (in 0.5 M HCl, pH 1.5) (Sigma Aldrich) for 24 hours.
Briefly, ethanol-fixed cells and matrix were stained for 1 h with 40 mM alizarin red-S (pH 4.2) and rinsed extensively with water.
Similar(56)
Thus, during a general H&E staining experiment, the nuclei of the cells are stained blue, whereas the cytoplasm and extracellular matrix are stained varying degrees of pink [ 57].
The pericellular matrix was stained by using the rat anti-heparan sulfate proteoglycan antibody (Millipore, Watford, UK) followed by the Alexa-Fluor-488 labeled anti-rat IgG (Life Technologies, Paisley, UK).
At 14 days after infection, mineralized matrix nodules were stained for calcium precipitation by means of Alizarin Red S staining, as described previously [24], [25], [27], [28], [29], [30], [31], [55], [61].
To study proteoglycan (PG) depletion from cartilage matrix, sections were stained with safranin O followed by counterstaining with fast green.
To study proteoglycan depletion from the cartilage matrix, sections were stained with safranin O. Depletion of proteoglycan was scored on an arbitrary scale of 0 to 3 ranging from normal fully stained cartilage to destained cartilage.
TEM analysis showed that the bacterial cells were surrounded by electron-dense fibrous matrices that were stained with ruthenium red (Figure 5).
After removal of the matrix, the slides were stained with Mayer's hematoxylin-eosin (HE).
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