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Decellularized rat blood vessel matrix was incubated with EDC cross-linked collagen and bound to NGF protein using the bifunctional coupling agent N-succinmidyl3- 2-pyridyldit-hio) N-succinmidyl3- 2-pyridyldit-hio
When a DNA-Cu2+ matrix was incubated in an aqueous hydroquinone or ascorbic acid solution, benzoquinone or dehydroascorbic acid was produced, respectively, by the oxidative effect of Cu2+ in the DNA-metal ion matrix.
A second seeded matrix was incubated outside the magnetic field.
Briefly, DNA embedded in agarose matrix was incubated at 37°C for 40 min with 2.5 units of S1 nuclease in 200 µl of 1x S1 buffer (Promega, Madison, USA).
For each experiment, one seeded matrix was incubated at 37°C/5% CO2 1.5 cm above a rare earth magnet of 12,100 Gauss (25 mm in diameter and 5.5 mm in thickness).
After washing with PBS, the matrix was incubated with 100 µl of LDL solution (0.5 mg/ml total protein in PBS) or 100 µl of HDL (1 mg/ml total protein in PBS).
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In this bioassay scheme, SPA RVC electrodes with avidin molecules immobilized on the SPA matrix were incubated with low quantities of b-BSA followed by incubation with avidinylated alkaline phosphatase (av-ALP).
Controls for each set of samples of bone matrix were incubated in equivalent volumes of phosphate buffer solution without the enzyme.
Briefly, 45 μl of matrix were incubated with 3 μg of anti-BAG3 (Ac-1), anti-SNAP-25, anti-syntaxin-1 or anti-FAK antibodies for 1 h at 4°C in continuous shaking.
Following PBS washes, matrices were incubated with 10 mg ml−1 DNase I (Roche) at 37 °C for 30 min.
After removal of P-TEFb and ATP, the matrices were incubated with HeLa nuclear extract in the presence of phosphatase inhibitor cocktail (Sigma) for 2 h at 4 °C.
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