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The drawback is that the properties of a matrix spiked with target DNA may differ from the properties of samples obtained from the field.
The optimization of this step was conducted by loading 0.07 g of matrix spiked with the target analytes (300 ng/mL) in toluene onto the SPE cartridges.
Artificial negative samples consisted of nontarget DNA and blank plant matrix and artificial positive samples consisted of plant matrix spiked with known quantities of target DNA (0.05 50 pg).
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Accuracy, repeatability and reproducibility of the proposed analytical procedure were tested in determination of Cr VI) in synthetic solutions and environmental water matrices spiked with the analyte.
Extraction recovery was evaluated for aqueous matrices spiked with stanozolol at pg/mL levels.
In case of a direct comparison of signals that are derived from different matrix batches spiked with analytes subsequent to the extraction, relative matrix effects can be addressed.
The basis of calculation of intra-day and inter-day precisions and accuracies was the analysis of RLMs matrix samples' spiked with MST at QC levels in 1 day and three consecutive days, respectively.
Human plasma or RLMs matrix was spiked with proper volumes of VNT WK2 (10 µg/mL) to produce two sets of twelve concentrations: 5, 10, 20, 30, 40, 50, 80, 100, 150, 300, 400 and 500 ng/mL.
In this study, the optimum conditions of extraction time and temperature were investigated on a 75 μm PDMS/carboxen fiber; 2 mL of the matrix sample spiked with target alkylpyrazines in 10 mL commercially available autosampler vial was used at the concentration of 500 ng/mL.
The blank matrix was spiked with corresponding amounts of standards and prepared as regular samples.
For the calculation of experimental sample concentrations, untreated udder matrix was spiked with the analyte [5 μg/g tissue].
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