Sentence examples for matrix solution in from inspiring English sources

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According to method A, the sample was mixed with synthetic matrix solution in a ratio of 1 2 and placed on a target plate.

Then 500 μL of 7.5 mg/mL α-cyano-4-hydroxycinnamic acid (CHCA) matrix solution in 50%acetonitrile/0.1%1 % trifluoroacetic acid was sprayed stepwise onto the tissue sections by using an Aztek airbrush model A4709 (Testor Corp., Rockford, IL), releasing short puffs of matrix solution interrupted with 3 5 s dwell time.

To characterize ionization properties, a dilution series of tamoxifen (0.001 10 µg/mL) was applied on a MALDI target plate and dried droplet sample spots were prepared by adding 1 µL of 3.5 mg/mL α-cyano-4-hydroxycinnamic acid (CHCA, from Sigma-Aldrich, St . Louis MO matrix solution in 50 % acetonitrile (AcN /0.1 % trifluoroacetic acid (TFA) to 1 µL tamoxifen solution.

Peptide digests were concentrated on C18-Zip Tips (Millipore) and eluted with 1.5 µl α-cyano-4-hydroxycinnamic acid matrix solution (in 50% acetonitrile, 0.05% trifluoroacetic acid) onto a MALDI-TOF target plate Opti-TOFF 384 well Insert, Applied Biosystems).

Column effluent was mixed 1∶1 with 2 mg/ml CHCA matrix solution in 60% acetonitrile and 0.1% TFA with 2 nM angiotensin II (MW 1045.53 Da) and 4 nM ACTH (MW 2464.19 Da) (Sigma, Zwijndrecht, The Netherlands) as standards for internal mass calibration.

Finally, the digested peptide samples were co-crystallized with an equal volume of saturated matrix solution (CHCA in 0.1% TFA in H2O/ACN [2:1]) on the MALDI sample target plate.

Drying of the plasma lysates after protein precipitation and subsequent reconstitution in a small volume of matrix solution resulted in poor crystallization of the samples and severe loss in sensitivity, which is in agreement with two previous reports [16], [22].

The voxels order aligned along diagonal direction was designed to allow fast parallel strategy for the lower and upper matrix solution involved in ADE scheme for irregular tissue.

Coverslips were coated with labelled matrix solution, fixed in 0.5% glutaraldehyde (Roth, Karlsruhe, Germany) and washed with 70% ethanol and medium.

Prior to MALDI-TOF/TOF analysis, 3 μl of each extracted peptide sample solution was mixed with 3 μl of alpha-cyano-4-hydroxycinnamic acid (Sigma-Aldrich, USA) matrix solution dissolved in 50% aqueous ACN containing 0.1% trifluoroacetic acid (Sigma-Aldrich, USA).