Exact(2)
For detection of AIV, 100 µL from 5 samples was pooled; RNA extracted from pools collected up to July 2012 was subjected to reverse transcription PCR (RT-PCR) to amplify 244 bp of the matrix segment of AIV, according to World Health Organization (WHO) protocol (12 ).
Although the resolution of the phylogeny inferred for the matrix segment is even lower, because of the short sequence length, the swIAVs from Brazil also are not monophyletic on the matrix tree, consistent with multiple introductions of pH1N1 into swine in Brazil (Technical Appendix Figure 7).
Similar(58)
Our Reality Matrix segments the market, positions early market leaders, and provides context for understanding the drivers.
All of these viruses had matrix segments of pH1N1 origin, and all other internal genes that could be sequenced were also of pH1N1 origin (Table 1).
All samples, control and experimental, were incubated at 15 C, and five matrix segments from each group were removed and washed several times in cold distilled water and implanted in allogeneic adult rats.
These matrices segment Pc in a straightforward way.
Indication elements consist of discrete light emitting diodes (LED), LED matrices, segment-type displays and small sized graphical visualization panels [14].
Based on the block-wise strategy, Farin et al. [19] used a block similarity matrix to segment the input video frames into foreground and background regions, which contain the block-wise temporal differences between any video frame pair.
The presence of influenza viruses was detected by quantitative real-time polymerase chain reaction (q-RT-PCR) targeting the Matrix gene segment, using a LightCycler 480 (Roche).
From a position-specific score matrix, any segment receives a score by adding the corresponding columns scores from the position-specific score matrix.
The primers and probe were designed based on the sequence homology of the matrix gene (segment 7) among different subtypes of influenza A strains of avian, human, swine and equine origin.
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