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To assess the position or critical residue that is important for M2 binding to CTCF, we made three point mutations (M2-Mut1, M2-Mut2, and M2-Mut3) according to position weight matrix score and performed EMSA assays (Fig. 2G).

We then grouped the predicted sites by matrix score, and computed the average tag coverage for each score class.

An index score for the severity of the amino acid change was calculated by adding the PAM250 substitution matrix score and the BLOSUM90 substitution matrix score.

Firstly, this change was reflected in both the Grantham matrix score and the more formal analysis as likely pathogenic mutation Group C in the ADPKD Mutation Database (http://pkdb.mayo.edu).

The UVs were further classified as high risk or low risk based on five methods: allele frequency, Polyphen algorithm, sequence conservation, Grantham matrix scores, and a combination of the Grantham matrix score and sequence conservation.

Similar(55)

For the community workshop and focus group discussion, a range of tools from participatory rural appraisal were used such as historical timelines, seasonal calendars, and matrix scoring and ranking.

In the current analyses, we classified BRCA1/ BRCA2 UVs using the four methods listed above and a combination of the Grantham matrix scores and sequence conservation.

Position weight matrix scores and location information were loaded into the University of California, Santa Cruz (UCSC) Genome Browser for visualization of YY1 location [ 36].

Different tools provide different analytic algorithms for individual motif detection, affinity binding, matrix scoring, and statistical testing of over-representation (see Methods).

To discriminate between these two hypotheses, we first we analyzed the correlation between weight matrix scores and ChIP-Seq tag coverage at the highest possible resolution.

The thresholds of the core score and matrix score values in TFBS scanning were 1.0 and 0.9, respectively, and the support and confidence values in co-occurrence analysis were set both at 90%.

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