Sentence examples for matrix rigidity on from inspiring English sources
Here we designed robust and reproducible laminin-polylysine grid micropatterns on cell culture substrates that have similar biochemical properties but a 100-fold difference in Young's modulus to investigate the role of the matrix rigidity on the formation and activity of cortical neuronal networks.
Therefore, we decided to quantify the effect of matrix rigidity on spreading of SCPs.
As illustrated, the assay system provides a rapid and reproducible method to assess the role of matrix rigidity on cell growth and survival.
Previous studies investigating the effects of matrix rigidity on cell migration, differentiation, and invasion have utilized in vitro cell culture on 2D model substrates, such as Matrigel™ [13], [14], crosslinked gelatin [15], and synthetic hydrogels [16], [17].
A schematic model showing the effect of matrix rigidity on CM cell fate.
Fibronectin-coated CytoSoftTM 35 mm biocompatible silicone dishes (Advanced BioMatrix) with elastic modulus of 0.5 and 64 kPa were used for studying the effect of matrix rigidity on stress fiber composition.
Nine of the cancer cell lines exhibited a dependence on matrix rigidity for growth, growing significantly better on stiff/rigid matrices than on the less rigid/soft matrices.
Remarkably, all of the cancer cell lines examined in this study were capable of proliferating on soft substrates, whereas normal fibroblasts, smooth muscle and epithelial cells exhibit a strict dependence on matrix rigidity for growth [25].
In particular, a large portion of the in vitro studies related to fibroblasts have been conducted on polyacrylamide coated with collagen I, where the effects of matrix rigidity or stiffness on fibroblast morphology and activation have been examined.
Cortical neurons and astrocytes respond strongly to changes in matrix rigidity when cultured on flexible substrates.
In addition, the differentiation of the stem cells through changes in gene expression was shown to depend on matrix rigidity [22].
