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We found the presence of Dp71, β-dystroglycan, nNOS, β-sarcoglycan, α/β syntrophin, α1-dystrobrevin and β-dystrobrevin in the nuclear matrix protein fractions and in situ nuclear matrix preparations from HeLa cells.
Calmodulin and two specific Ca2+-dependent calmodulin-binding proteins were found to be associated with matrix preparations.
We also addressed the requirement for Topoisomerase II, a major component of nuclear matrix preparations, for maintenance of chromosome architecture.
Preparations using lithium-3,5-iodosalicylate (LIS) extraction are referred to as nuclear scaffolds, while extraction with 2 M NaCl are referred to as nuclear matrix preparations.
Nuclear matrix preparations were done as described [ 43].
Small-scale studies have suggested that NaCl extracted nuclear matrix preparations will identify MARs that are associated with transcriptionally active regions (28).
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The MPSMs were contained in a glass vial, placed on a rotating shaker, and sterilized under UV light (260-nm wavelength) for 60 min. Matrix Preparation: Following the sterilization process, the matrix was prepared by mixing MPSM (25% w/v) in DD-pMEO2MA polymer (4% w/v) (predissolved in cell culture media).
The optimal conditions for nanocomposite matrix preparation were studied.
using of innovative matrix preparation technology to increase pore structure, especially pore volume, and not effect on the attrition of catalyst; 3.
Rat bladder epithelial cell line 804G was cultured in minimum essential medium supplemented with 10% FCS and employed for LM5 rich matrix preparation [39].
Subsequently, supernatants were mixed with matrix solution (1∶2, v/v; for matrix see Section "matrix preparation"), 0.75 µL matrix/sample mixture was deposited on three positions on the target plate and was dried at room temperature.
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