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Matrix mineralization is considered a functional in vitro endpoint reflecting advanced cell differentiation.

During the second maturation phase of MC3T3-E1 cells occurring from weeks 2 4, matrix mineralization is observed [38, 39].

Extracellular matrix mineralization is one of the markers of osteoblastic maturation.

A previous study reported that a relatively less extensive area of matrix mineralization is often seen in higher-grade chondrosarcomas on plain radiography and CT [ 18].

Expression of ALP, OC, and Runx2 is a marker of osteoblastic differentiation, while matrix mineralization is a hallmark of the osteoblast phenotype [ 14, 15].

The enamel matrix mineralization is influenced by globular proteins such as amelogenin and enamelin [ 19, 20] which constitute a small fraction of the final mineralized matrix.

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Matrix mineralization was assessed by Alizarin Red S staining.

Consequently, a robust matrix mineralization was achieved, covering >90% of the culturing surface area.

The matrix mineralization was approved by alizarin red and the treated cultures with osteogenic media and BMP2 were positive for osteopontin and osteocalcin antibodies.

When cell differentiation and matrix mineralization were evaluated after days 7 and 28, the MWCNT-COOH group showed increased cell differentiation and matrix mineralization, when compared to the MWCNT group.

Type-I collagen (the major protein of the osteoid matrix) as well osteocalcin and osteopontin (proteins involved in matrix mineralization) are also produced in higher quantity (Gevers & Dequeker 1987, Hilal et al. 1998, Sanchez et al. 2008).

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