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Bone matrix mineralization by BMP2-iPSC-MSCs was 1.8 times that of iPSC-MSCs at 21 d.
Furthermore, the biofunctionalized HA hydrogels promote the alkaline phosphatase activity, type I collagen deposition, and matrix mineralization by the seeded hMSCs under both in vitro and in vivo condition.
It is degraded by cathepsin B to an acidic, negatively charged ASARM peptide which inhibits osteoblast matrix mineralization by directly binding to HA [14,15,18].
These studies pointed out that osteoblasts change their morphology, gene expression, and matrix mineralization by either introducing surface topography on biomaterials or mechanical stimulation [ 119, 120].
This effect shows that bortezomib promotes matrix mineralization by osteoprogenitor cells through the stabilization of β-catenin and induction of TCF transcriptional activity [ 77].
This fragment contains the ASARM peptide; thus, we next determined the role of the ASARM peptide in chondrocyte matrix mineralization by examining the mineralization capability of ATDC5 cells in response to MEPE-ASARM peptides.
Similar(54)
We found that matrix mineralization assessed by alizarin red staining was remarkably promoted by the combination of BMP and miR-548d-5p miR-548d-5p miR-548d-5pparate treatment).
Thus the tailored surface of genetically engineered PVX forms fibril-like nanostructures which enables enhanced focal adhesion-dependent cell adhesion, and matrix mineralization verified by Alizarin.
Noggin siRNA treatment successfully down-regulated cellular Noggin protein levels and enhanced BMP signal activity which in turn resulted in significantly increased osteoblast differentiation and extracellular matrix mineralization evidenced by histological assessments.
Subsequently, the matrix mineralization occurs by deposition of hydroxyapatite [ 169, 170].
Matrix mineralization obtained by the use of depleted MSCs was higher than that for MSCs in PL medium (p < 0.05) and appears to be higher in osteogenic medium (missing statistical significance).
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