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The absence of DNA in the matrix lysates further confirmed that approximately 99% of the DNA was removed by the de-cellularisation process.
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Since the identification of CPS1 as a SIRT5 deacetylation target from mouse mitochondria matrix lysates, most studies have focused on the function of SIRT5 in mitochondria.
Or as an alternative, matrix, lysate or tissue with low target levels compared to matrix, lysate or tissue with artificially increased target levels.
This may translate into comparing matrix, lysate or tissue containing endogenous target levels with matrix, lysate or tissue containing knocked-down or knocked-out target levels.
A staff training matrix identifies further training required, meaning staff are fully qualified at all times.
The compromise matrix was further analyzed by principal components analysis.
This matrix was further normalized as follows.
This modified matrix is regarded as the initial matrix for further process of optimization.
This composite score matrix was further used to calculate a Euclidean distance matrix.
This matrix is further normalized according to the node degree.
Cell lysates were further processed with sonication and the protein concentration determined by BCA assay (Thermo).
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