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This study evaluates the effects of SG decellularization and subsequent cryopreservation on the extracellular matrix integrity of allograft heart valves.
Objective of this study was to investigate the impact of different decellularization protocols on extracellular matrix integrity of xenogeneic tissue by means of multiphoton femtosecond laser scanning microscopy, biochemical and histological analysis.
Thus, it was necessary to develop techniques of decellularizing the organ structure and removing the residual DNA, preserving the extracellular matrix integrity of an organ.
In addition, the matrix integrity of the cartilage seemed to be largely unaffected during culture, except for the well-known detachment of the superficial lamina splendens.
In vitro, UTE-T2* values have been shown to reflect collagen matrix integrity of human articular cartilage explants as determined by polarized light microscopy [ 41].
The matrix integrity of the cartilage seemed to be largely unaffected during the whole culture period, except for a detachment of the superficial layer, presumably the lamina splendens, from the underlying tissue and a subsequent demasking of cartilage matrix structures.
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For examination of matrix integrity, a subset of coated transwells were biotinylated under sterile conditions, using 20 μg/ml EZ-Link Sulfo-NHS-LC-LC-Biotin (Pierce, Rockford, IL) in 50 mM sodium bicarbonate, pH 8.3 for 2 hrs, and subsequently quenched using 50 mM Tris-HCl, pH 7.5.
Progression of tendinopathy is dependent on extracellular matrix integrity and remodeling of the tendon is a common feature of tendinopathy [ 24].
While in some animals IL-1 ra did show a chondroprotective effect with preservation of cartilage matrix integrity, in the majority of animals, IL-1 ra treatment failed to provide any disease-modifying effect.
Osteogenic protein 1 (OP-1), a member of the bone morphogenetic protein (BMP) family, is expressed by human adult articular chondrocytes and plays a crucial role in the maintenance of cartilage matrix integrity and the promotion of repair processes [ 8, 9].
RECK has been shown in studies of wound healing and cancer metastasis to be a key regulator of the extracellular matrix integrity and an inhibitor of angiogenesis [11], [17].
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