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A special feature of SinR of relevance to this investigation is that the expression of matrix genes is hypersensitive to small perturbations in the level of the protein (Chai et al., 2011).
Previous studies have shown that in TNFα-stimulated chondrocytes inhibition of various extracellular matrix genes is directly related to binding of Egr-1 to specific regions in the promoter [67].
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A limitation of our study is that a role for the miR-29 and miR-15 family MREs in the CDS of elastin and other matrix genes was not demonstrated in an in vivo model system.
Cartilage-specific matrix genes were found to be modulated by rhOP-1 treatment.
However, the general histological structure and expression of matrix genes was not affected (Fig. 6c).
Microaarray analysis provided evidence that 27 extracellular matrix genes were differentially regulated in diabetes.
These results suggest that the extracellular matrix genes are new therapeutic targets for invasive tumors.
In contrast, the other three specific eggshell matrix genes were expressed only in the uterus as expected.
We found that downregulation of several mitochondrial genes and upregulation of many extracellular matrix genes was a common feature of all trisomic hearts.
Unfortunately many of these key matrix genes are expressed in a variety of mesenchymal tissues and therefore may not be sufficiently discriminatory.
Representative images are shown in Figure 2. Chondrocyte differentiation and the expression of cartilage matrix genes are in part regulated by Sox transcription factors [ 42].
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