Sentence examples for matrix gene segment from inspiring English sources

The presence of influenza viruses was detected by quantitative real-time polymerase chain reaction (q-RT-PCR) targeting the Matrix gene segment, using a LightCycler 480 (Roche).

The primers and probe were designed based on the sequence homology of the matrix gene (segment 7) among different subtypes of influenza A strains of avian, human, swine and equine origin.

However, screening for other virus subtypes by virus isolation, or targeting the matrix gene segment in molecular-based diagnostics, will simultaneously enhance our ecologic, epidemiologic, and virologic understanding of AIV.

In addition, the splicing pattern of the matrix gene segment and the reduced 5-N-acetyl binding pocket in the hemagglutinin-esterase (HE) protein of D/bovine/France/2986/2012 was similar to that of C/OK and different from that of human influenza C virus, confirming the similarity of D/bovine/France/2956/2012 and the newly described swine and bovine US influenza D virus strains.

For the amplification of the nonstructural and matrix gene segments, primers in the 5′ and 3′ noncoding region of the RNA segment were used to amplify the complete coding sequence to be used for direct sequencing.

Primers and probes were designed to correspond to conserved regions of the FluA matrix (M) gene segment.

The isolates were also tested for resistance to amantadine by an in-house designed PCR (protocol avain-house designed) and nucleotide sequencing of the matrix (M2) gene segment.

Swab specimens were screened in pools of 4 by using a real-time reverse transcription PCR for the matrix (M) gene segment (6 ).

Next, we performed sequence alignment and pairwise comparison of the full gene segments of the 37 H9N2 viruses with respect to each other, and a percent identity matrix was generated for each gene segment.

Samples were tested by using a generic real-time PCR specific for the genomic RNA segment 7 (matrix gene) of the influenza A virus provided by the National Centre for Foreign Animal Disease (Winnipeg, Manitoba, Canada) (3 ) and an H1 differential PCR (6 ) provided by the National Microbiology Laboratory (Winnipeg, Manitoba, Canada).

We constructed a nearly complete generic-level phylogeny of the Mustelidae using a data matrix comprising 22 gene segments (~12,000 base pairs) analyzed with maximum parsimony, maximum likelihood and Bayesian inference methods.