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Q: The matrix gene?
For the matrix gene, after the RT-PCR assay, a subsequent nested PCR was carried out.
The sequence diversity of this virus, even in the conserved matrix gene M1, makes primer and probe designs challenging.
In this study several commonly used scaffold materials for cartilage tissue engineering were evaluated with respect to their influence on matrix gene expression.
These results demonstrated that the knitted silk + collagen sponge scaffold improves structural and functional ligament repair by regulating ligament matrix gene expression and collagen fibril assembly.
We use plasmids containing the influenza A matrix gene and the porA and ctrA genes of Neisseria meningitidis as targets for developing the system.
Transforming growth factor-β is essential in regulating extracellular matrix gene expression and is dependent on Smad3, an intracellular mediator/transcription factor.
A RRT-PCR system targeting the matrix gene was used for screening and quantification [44].
The RPM-Flu microarray includes four influenza virus matrix gene detector tiles, representing matrix gene sequences from selected reference strains of A/H1N1(human), A/H3N2(human), A/H5N1 avian) and B(human) types and subtypes.
AM Probe: Taqman® probe that hybridizes to a specific target sequence on the Influenza A matrix gene, 10 pmol/µl.
The presence of influenza viruses was detected by quantitative real-time polymerase chain reaction (q-RT-PCR) targeting the Matrix gene segment, using a LightCycler 480 (Roche).
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