The scan parameters were as follows: TR = 2000 ms, TE = 30 ms, flip-angle = 90°, 64×64 matrix, field of view 220 mm, 26 parallel axial slices covering the whole brain, 6 mm thickness.
First, structural images of the brain were obtained by means of a T1-weighted MPRAGE sequence: 256×256 matrix; field of view (FOV) = 256 mm; 192 1-mm sagittal slices.
Individual anatomical data were acquired with a T1-FFE sequence (TR 30 ms, TE 4.5 ms, flip angle 30°, 70 slices, 256 × 256 matrix, field of view 256 mm × 256 mm, slice spacing 2 mm).
Briefly, we applied a T1-weighted, water-suppressed, gradient echo technique (repetition time, 80 ms; echo time, 6.11 ms; 512 × 512 matrix; field of view, 500 × 500 mm; slice thickness, 10 mm; interslice gap, 10 mm) to image abdominal fat during repetitive breath-holds.
For the movie study whole brain volumes of 48, 3-mm thick sagittal images (time repetition [TR] = 3000, time echo [TE] = 30 ms, flip angle = 90, 64 × 64 matrix, field of view [FOV] = 192 mm × 192 mm) were obtained every 3 s continuously through each half of the movie (1101 volumes for part 1, 1110 volumes for part 2).
For each subject 2 × 125 whole brain volumes were acquired using an echoplanar imaging (FEE-EPI) sequence (TR 2600 ms, TE 50 ms, flip angle 90°, interleaved scanning in ascending slice order, 22 slices, 64 × 64 matrix, field of view 192 mm × 192 mm, slice spacing 5 mm including 0.5 mm gap).
The T2-weighted images were acquired with a 320 × 256 matrix, field-of-view = 91 × 91 mm, repetition time = 4500 msec, echo time = 158 msec, and a total scan time of 6 min 12 sec.
T1-weighted images were acquired with a 384 × 307 matrix, field-of-view = 70 × 70 mm, repetition time = 507 msec, echo time = 17 msec, and a total scan time of 3 min 42 sec.
Fluorescence emissions were recorded within 1 Airy disk confocal pinhole opening and images at a 0. 238 µm (x, y) pixel size were obtained for each case in 1024×1024 matrices (fields of 158,94 µm2).
32 T2*-weighted echo-planar images (EPI) per volume with blood oxygenation level-dependent (BOLD) contrast were obtained (matrix 64×64, field-of-view 224×224 mm, spatial resolution: 3,5×3,5×4 mm, TE = 30 ms, TR = 2000 ms, flip angle 80°).
High matrix, reduced field-of-view imaging is also important for resolution of prostate tumors.
