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However, this type of culture surface can be modified and coated with matrix extracts from which the isolated cells originate, thus providing a biomimetic microenvironment coupled with mechanical stimulation [ 16].
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Second, applying KECA method with Gaussian kernel function on the feature matrix extracted from the de-noised signal.
The eigenvalues and mode shapes of the single plate are obtained from the determinant of a system matrix extracted from the entire system matrix equation.
Starting from the synonym/antonym matrix extracted from the widely-employed English thesaurus of Microsoft Word (MS English), optimization converges to a definite stable state that is macroscopically independent of the initial random conditions (details in the Materials and Methods section above).
The raw data matrix extracted from Beadstudio was uploaded in Bioconductor R version 2.9.1 for downstream analysis.
Endothelial tube formation was assessed using Matrigel™, a solubilized extracellular basement membrane matrix extracted from the Engelbreth-Holm-Swarm mouse sarcoma, as described previously [ 53].
The raw data matrix extracted from Beadstudio was uploaded into GeneSpring GX 7.3.1 (Silicon Genetics, Redwood City, CA) software for downstream analysis.
The Karhunen Loeve Transform (also known as the Principal Components Transform) and its inverse (IKLT) may then be defined as (4) KLT = V × I, IKLT = V T × KLT, with V : eigenvector matrix extracted from I V T : eigenvector transpose matrix.
It has previously been shown that matrix extracts obtained from murine mammary glands at various developmental stages, differently influence MDA-MB-231 invasion [ 63], and that both age and reproductive state of stroma, can differentially affect breast tumor development [ 64].
Examples for approaches in this field are the coating of polymers or metals with extracellular matrix extracts produced from sarcoma cells which are harvested and decellularized,[ 1] or the production of ECM on an artificial surface by cells which are removed prior to application.
Figure 9 Coordinate matrices extracted from sample 1 (Figure4) and sample 2 (Figurespectivelytively.
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