Also, several extraction solvents and the matrix effect were evaluated.
Key parameters, such as the type and volume of the chelation, extraction and dispersive solvent, aspiration speed, sample pH, salt effect and matrix effect, were thoroughly investigated.
The results; recovery of plasma analyte after extraction procedures (> 84.89%), linearity, accuracy and precision alongwith stability (bench-top, long term, freeze thaw stability and post processing stability) and matrix effect, were found in acceptable range.
Extraction and matrix effect were evaluated by employing Matuszewski method.
From this experiment, the linearity, repeatability, limit of detection, carry-over and matrix effect were also determined.
This analytical method (with enzymatic degradation) was validated by assessing selectivity, linearity, accuracy, precision, sensitivity, stability, matrix effects, and extraction recovery; data are summarized in Table 2.> As it can be seen, adequate stability, analytical precision accuracy, extraction recovery, and matrix effect were obtained.
