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Evaluation of the matrix effect showed that the method is not affected by relative matrix effects.
The extensive investigation of matrix effect showed that LLE yields cleaner extracts than the SPE.
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Precision and accuracy of the assay are shown in Table 1 and recovery and matrix effects are shown in Table 2.
In minced meat, PLS models showed reduction in the prediction capabilities caused by a matrix effect, nevertheless the system still shows good accuracy and precision in the determination of concentration levels of these anions.
he results showed the matrix effect for cathinone at lowest tested concentration (10 ng/ml) was significantly reduced from 210.9 to 133.7%, but not for mephedrone (from 196.8 to 191.9%) by using standard addition method.
The results showed the matrix effect for cathinone at lowest tested concentration (10 ng/ml) was significantly reduced from 210.9 to 133.7%, but not for mephedrone (from 196.8 to 191.9%) by using standard addition method.
Evidently, due to matrix effects, these values show a decrease in sensitivity depending on the matrix and toxin.
Our experiments of other baby food products indicated more matrix effects (data not shown); however, those matrix effects can be further corrected by the use of isotope dilution in IC-MS/MS analysis.
SF samples needed to be diluted 1 50 or more for a linear recovery at different dilutions; at dilutions below 1 50 the signal was reduced due to unknown matrix effects (results not shown).
During method development, different stationary phases (Agilent ZORBAX Poroshell 120 EC-C18 and ZORBAX RRHD Eclipse Plus C18) and column lengths (50 and 100 mm), as well as column temperatures, flow rates, gradients and injection volumes, were tested with regard to resolution, peak intensity and shape as well as matrix effects (data not shown).
Nevertheless, the result shows that the matrix effect is not a major source of error in our experiments.
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