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The aim of this part of the work has been to characterize in Gran Sasso groundwater samples the matrix concentrations of the elements Ba, Sr, Ce, La, and Nd, which are responsible for interferences, and to evaluate if, after a pre-concentration, these interferences could really be observed, and what would be their impacts on the Ra peak.
Thus, one would anticipate correlations between matrix concentrations of growth factors and functional properties of bone.
Matrix concentrations of TGF-β2 and IGF-I were measured by ELISA.
These modulus and strength data were used to correlate mechanical properties with matrix concentrations of growth factors in our study.
Increased local matrix concentrations of growth factors are associated with poor biomechanical and architectural properties of tibial cancellous bone.
We found higher matrix concentrations of TGF-β2 were associated with higher Tb.Sp and VMExp/σapp for pooled data and within subjects.
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The temporal dependence of the matrix concentration of each RE was utilized to determine its solubility in Al.
We evaluated key operating parameters of the assay such as cell number, percentage of serum matrix, concentration of the therapeutic drug, concentration of the cross-linker, length of various incubation steps, cell age, interval between cell subculture and bioassay time, and detection equipment.
Decreasing cancellous bone strength and stiffness with increasing matrix concentration of IGF-I has been reported previously [ 18].
Our previous work demonstrated the matrix concentration of IGF-I in cancellous bone is negatively correlated with the bone volume fraction, strength, and stiffness of cancellous bone from proximal tibiae of human male cadavers within subjects [ 18].
Similarly, a higher matrix concentration of IGF-I was associated with lower stiffness, strength, BV/TV and Tb.Th and with higher BS/BV, Tb.Sp, VMExp/σapp and VMCOV for pooled data and within subjects.
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