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As the matrix compound and other endogenous isobaric molecules often share nominal mass with the endogenous metabolites of interest, researchers cannot simply interpret the obtained ion intensity as being the analyte concentration.
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Next the relationship between individual matrix compounds and FEV1 was shown to be significant (Table 3).
Acetone purification as well as paper chromatography leave the sample with quite some matrix compounds and salts; therefore, SPE methods may be preferred.
Two wash buffers (PBS and PBST) were tested to remove matrix compounds and PBST was found most suitable as wash buffer and the same buffer was used to resuspend the beads.
Both this barrier function and epithelial turnover are strongly dependent on paracrine and exogenous factors, on extracellular matrix compounds and on the epithelial mesenchymal cell cell interaction in the intestinal mucosa.
A total of 15% (v/v) methanol in water, followed by n-hexane allowed for the removal of a substantial amount of matrix compounds and to wash out the non-reacted quenching agent (undecanal) from the cartridges.
Extended exposure times in combination with continuous heating and open-air conditions increased the risk for artificial product formation especially in the presence of complex mixtures of reactive analytes and matrix compounds and might promote oxidation of volatile organic compounds [ 32].
The increase in lightness values in the film incorporated with CLC was probably due to the light scattering effect of the matrix compound of CLC and gelatin in the film.
In this process, cells capable of producing tissue interact with chemotactic factors to form bone. Firstly, osteoblasts secrete substances to form osteoid tissue or immature bone, a nonmineral matrix compound of collagen [ 168] and glycosaminoglycans.
The whole mouse eye, eyelid (2 months old, n = 6) and human sclera (n = 3) were embedded in OCT (frozen tissue matrix) compound at −20°C for 1 hour.
For very intense matrix ions, a number of such artifact peaks were present and when these happen to correspond with the exact mass of a target analyte eluting closely to the matrix compound, a false positive was obtained.
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